Used in biomedical research to measure levels of 6-keto-prostaglandin F1α, a stable metabolite of prostacyclin, in biological samples such as plasma, serum, or tissue homogenates. This kit helps assess endothelial function and vascular inflammation in studies related to cardiovascular diseases, thrombosis, and atherosclerosis. It is also applied in pharmacological research to evaluate the effects of drugs on prostaglandin pathways. The assay enables high-throughput, quantitative analysis with high sensitivity and specificity, making it suitable for both in vivo and in vitro experimental models.