Used in biomedical research to detect and quantify Kidney Injury Molecule-1 (Kim-1) in rat kidney tissue or biological fluids such as urine and serum. This ELISA kit is specifically designed for early detection of acute kidney injury (AKI) in preclinical studies. It enables researchers to assess renal tubular damage in experimental models, particularly in toxicology and drug development studies where nephrotoxicity is a concern. The assay provides a sensitive and specific measurement of Kim-1, a biomarker that is significantly upregulated in injured renal epithelial cells, allowing for non-invasive monitoring of kidney damage progression and response to therapeutic interventions. Widely used in pharmaceutical research, environmental toxicity testing, and mechanistic studies of kidney disease.