Used in biomedical research to quantify leucine levels in mouse biological samples such as serum, plasma, or tissue homogenates. This assay helps in studying amino acid metabolism, nutritional status, and metabolic disorders in mouse models. It is also applied in research on insulin signaling, muscle protein synthesis, and liver function due to leucine's role in regulating these pathways. The kit enables high-throughput, sensitive, and specific detection, making it valuable in preclinical studies and experimental therapeutics.