Used in biomedical research to detect and quantify levels of creatine kinase MB isoenzyme, a biomarker associated with myocardial injury. The kit enables accurate measurement of CK-MB in serum or plasma samples, aiding in the assessment of heart damage, particularly in experimental models involving mice. It is commonly applied in studies related to cardiac function, ischemia-reperfusion injury, and evaluation of potential cardioprotective therapies. The ELISA format allows for high sensitivity and specificity, making it suitable for research settings requiring reliable data on cardiac biomarker expression.