Used in biomedical research to quantify metallothionein protein levels in human tissue extracts, cell lysates, and biological fluids. Enables studies on metal homeostasis, oxidative stress response, and detoxification mechanisms. Commonly applied in environmental toxicology to assess exposure to heavy metals such as cadmium, mercury, and lead. Supports research in diseases linked to metal dysregulation, including neurodegenerative disorders, cancer, and liver disease. Provides a reliable, sensitive method for high-throughput screening in clinical and experimental settings.