Used in biomedical research to quantify myosin heavy chain (MHC) protein levels in biological samples such as muscle tissue homogenates, cell lysates, and serum. This kit is essential for studying muscle development, regeneration, and function. It supports research in neuromuscular diseases like muscular dystrophy, sarcopenia, and myositis by enabling accurate measurement of MHC expression changes in response to disease, injury, or therapeutic interventions. Also used in exercise physiology to assess muscle fiber type transitions and adaptation following training or disuse. The assay provides high specificity and sensitivity, making it suitable for preclinical studies and biomarker validation in experimental models.