Used in biomedical research to detect and quantify creatine kinase isozyme MB levels in rat samples, particularly in studies related to myocardial injury or cardiac damage. Helps researchers assess heart tissue damage in experimental models, such as those involving induced ischemia, drug toxicity, or cardioprotective interventions. The kit enables accurate measurement of CK-MB, a biomarker associated with heart muscle stress, supporting preclinical studies in cardiology and pharmacology. Widely applied in laboratory settings for evaluating the efficacy of therapeutic agents on cardiac health in rat models.