Used to measure endothelial nitric oxide synthase (eNOS) levels in mouse tissue samples, particularly in cardiovascular and vascular biology research. Helps assess endothelial function and nitric oxide production in studies related to hypertension, atherosclerosis, and ischemia-reperfusion injury. Commonly applied in preclinical studies using mouse models to evaluate the impact of drugs, genetic modifications, or disease states on vascular health. Provides a quantitative and sensitive method to detect eNOS protein concentration in cell lysates or tissue homogenates, supporting research into signaling pathways involved in vasodilation and inflammation.