Used in biomedical research to quantify neprilysin (NEP) activity in mouse tissue samples and biological fluids. Helps study the role of enkephalinase in pain modulation, neurodegenerative diseases, and opioid peptide regulation. Commonly applied in neuroscience and immunology studies to assess enzyme levels in experimental models of inflammation, Alzheimer’s disease, and chronic pain. Enables researchers to evaluate the effectiveness of NEP inhibitors and their impact on peptide metabolism in vivo.