Used in biomedical research to measure endothelial nitric oxide synthase (eNOS) levels in cell cultures, tissue extracts, and biological fluids. Helps assess endothelial function and nitric oxide production in studies related to cardiovascular health, hypertension, atherosclerosis, and vascular inflammation. Commonly applied in preclinical drug development to evaluate the impact of therapeutic compounds on endothelial signaling pathways. Also utilized in clinical research to investigate eNOS dysregulation in metabolic disorders, diabetes, and ischemic conditions. Provides a reliable, high-throughput method for quantifying eNOS protein concentration with high sensitivity and specificity.