Used in biomedical research to quantify endothelial nitric oxide synthase (eNOS) levels in cell lysates, tissue extracts, and biological fluids. Enables assessment of endothelial function and nitric oxide pathway activity in studies related to cardiovascular health, atherosclerosis, hypertension, and diabetes. Supports investigation of drug effects, oxidative stress, and inflammatory responses on vascular endothelium. Provides a reliable, high-throughput method for detecting eNOS protein concentration in experimental models and clinical samples.