Used in biomedical research to quantitatively measure the levels of human 5'-nucleotidase enzyme in biological samples such as serum, plasma, or cell lysates. This ELISA kit is particularly useful in studies related to purinergic signaling, immune function, and liver or vascular health, as 5'-NT plays a key role in nucleotide metabolism and adenosine production. It supports research into inflammatory conditions, cancer, and metabolic disorders by enabling accurate detection of enzyme concentration in experimental models. The assay is widely applied in clinical laboratories and pharmaceutical research for biomarker validation and monitoring disease progression.