Used in biomedical research to quantify N-acetyl-β-D-glucosamine levels in rat biological samples such as serum, urine, or tissue homogenates. Helps assess kidney function, particularly proximal tubular damage, as NAG is a lysosomal enzyme released during renal injury. Commonly applied in toxicology studies to monitor nephrotoxic effects of drugs or environmental agents. Also used in diabetes research due to associations between elevated NAG excretion and early diabetic nephropathy. Provides a sensitive, specific, and high-throughput method for enzyme level detection, supporting both clinical and experimental investigations into renal health and disease progression.