Used in biomedical research to detect and quantify 5-nucleotidase enzyme activity in rat tissue samples, particularly in studies related to liver function, cholestasis, and hepatobiliary diseases. The ELISA kit enables high-throughput analysis in laboratory settings, supporting investigation of enzyme expression levels under experimental or pathological conditions. Commonly applied in toxicology studies, drug development, and metabolic research where changes in nucleotidase activity serve as a biomarker for cellular stress or membrane integrity.