Used in biochemical and medical research to detect and quantify levels of acetylserotonin methyltransferase (ASMT) enzyme activity in plant and sometimes animal samples. Helps researchers study melatonin biosynthesis pathways, as ASMT is a key enzyme in the production of melatonin, which regulates circadian rhythms and seasonal responses. The ELISA format allows for high-throughput analysis in studies related to plant stress responses, photoperiodism, and developmental physiology. Also utilized in comparative studies between plant and mammalian melatonin synthesis, supporting advances in agricultural science, chronobiology, and neuroendocrine research.