Used in biomedical research to detect and quantify protein phosphatase activity in cell lysates, tissue extracts, and biological fluids. Enables study of cellular signaling pathways, particularly those involving phosphorylation and dephosphorylation processes linked to diseases such as cancer, diabetes, and neurodegenerative disorders. Supports high-throughput screening in drug development to assess compound effects on phosphatase regulation. Provides a reliable, sensitive method for monitoring changes in protein phosphatase levels under experimental conditions.