Used in biomedical research to detect and quantify specific proteins, hormones, or antibodies in biological samples such as serum, plasma, or cell lysates. Commonly applied in studies related to immune response, disease biomarker discovery, and drug development. The kit enables high-sensitivity detection through enzyme-linked immunosorbent assay (ELISA) methods, where peroxidase enzyme catalyzes a colorimetric reaction for measurable signal output. Widely used in preclinical research involving rat models to assess protein expression levels in a reliable and reproducible manner.