Used in biomedical research to quantify total protein concentration in human biological samples such as serum, plasma, and cell culture supernatants. Enables researchers to assess protein expression levels, monitor disease progression, and evaluate the effectiveness of therapeutic interventions. Commonly applied in clinical laboratories and life science studies focusing on inflammation, liver function, nutritional status, and immune response. The kit provides a sensitive and specific method for detecting both albumin and globulin fractions, supporting accurate normalization in experimental assays.