Used in biomedical research to quantify levels of Human Topoisomerase II (TOP2) protein in cell lysates, tissue extracts, or biological fluids. This kit enables researchers to study the expression and regulation of TOP2, an enzyme critical for DNA replication, transcription, and chromosome segregation. It is widely applied in cancer research, as TOP2 is a target for chemotherapeutic drugs like etoposide and doxorubicin. The assay helps evaluate TOP2 levels in tumor samples, assess drug efficacy, and investigate mechanisms of drug resistance. Also used in toxicology studies to monitor DNA damage response and in basic molecular biology to explore enzyme dynamics during the cell cycle.