Used in biomedical research to detect and quantify levels of human topoisomerase enzymes in cell lysates or tissue samples. Widely applied in cancer studies, as topoisomerases are targets of several chemotherapeutic drugs. Helps assess enzyme expression and activity in response to drug treatments, DNA damage, and cellular stress. Also utilized in drug development and screening to evaluate inhibitor efficacy and monitor biomarker changes in diseases involving DNA replication and repair dysfunction.