Used in biomedical research to quantify mTOR protein levels in cell or tissue lysates, this ELISA kit enables detailed study of mTOR signaling pathway activity, which plays a central role in regulating cell growth, proliferation, survival, and metabolism. Researchers apply it in cancer studies to assess pathway dysregulation and evaluate responses to therapeutic agents such as rapamycin analogs. It is also used in metabolic disease research, aging studies, and immunology to monitor how nutrients, growth factors, and stress signals influence mTOR activation. The assay provides a sensitive and specific means to measure phosphorylated or total mTOR, supporting drug development and mechanistic investigations in preclinical models.