Used in biomedical research to measure levels of coagulation factor V in rat plasma or serum samples. Enables researchers to study blood clotting mechanisms, evaluate coagulation disorders, and assess the effects of drugs or genetic modifications on the coagulation pathway. Commonly applied in studies related to thrombosis, hemorrhage, liver function, and inflammation. Provides a sensitive and specific method for quantifying factor V activity indirectly through antigen detection, supporting preclinical research in hematology and pharmacology.