This chemical is primarily used in biomedical research to study angiogenesis and vascular endothelial growth factor signaling pathways. It serves as a functional assay system for screening potential anti-angiogenic compounds, especially in cancer drug development. The construct enables detection of VEGFR2 activation through NF-AT-mediated reporter gene expression in HEK293 cells, allowing high-throughput evaluation of inhibitors or modulators targeting VEGFR2 activity. It is also employed in toxicity studies and mechanistic investigations of vascular-related diseases.