Rapid Endonazyme XcmI is a high-efficiency restriction enzyme used in molecular biology for precise DNA cleavage. It is commonly applied in DNA cloning, restriction mapping, and genetic engineering workflows where specific DNA fragmentation is required. Due to its rapid digestion capability, it significantly reduces reaction time—often completing digestion in 5–15 minutes—making it ideal for high-throughput applications and time-sensitive experiments. It functions reliably under a universal buffer system, allowing simultaneous use with other restriction enzymes in double or multiple digests without compromising activity. This feature enhances cloning efficiency and simplifies experimental design. The enzyme is also suitable for diagnostic applications involving DNA fingerprinting and genotyping, where reproducible and clean cuts at specific recognition sites are critical. Its stability and consistency make it a preferred choice in both research and industrial molecular biology settings.