Fluorescein5(6)-isothiocyanate

90%, 5- and 6-isomer mixture

  • Product Code: 127131
  Alias:    Fluorescein isothiocyanate; 5(6)-fluorescein isothiocyanate, 4'(or 5')-fluorane isothiocyanate
  CAS:    27072-45-3
Molecular Weight: 389.38 g./mol Molecular Formula: C₂₁H₁₁NO₅S
EC Number: 248-207-7 MDL Number: MFCD00005063
Melting Point: >360 °C(lit.) Boiling Point:
Density: Storage Condition: 2-8°C
Product Description: Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology for tagging proteins, antibodies, and other biomolecules. It enables visualization and tracking of these molecules in techniques like fluorescence microscopy, flow cytometry, and immunoassays. Its isothiocyanate group reacts with primary amines, forming stable thiourea bonds for covalent attachment. Commonly applied in diagnostics, cellular imaging, and research to study molecular interactions, localization, and dynamics in biological systems.
Product Specification:
Test Specification
Appearance Light Yellow To Yellow To Orange To Brown Powder To Crystal
Purity (%) 89.5-100
Infrared Spectrum Conforms To Structure
NMR Conforms To Structure
Solubility (1mg/mL, Acetone) Very Faint Yellow To Yellow And Very Faint Green-Yellow To Green-Yellow Liquid
Carbon (C) 54.1-71.0
Nitrogen (N) 3.0-4.0
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
0.050 10-20 days $31.07
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0.250 10-20 days $85.73
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1.000 10-20 days $301.24
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Fluorescein5(6)-isothiocyanate
Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology for tagging proteins, antibodies, and other biomolecules. It enables visualization and tracking of these molecules in techniques like fluorescence microscopy, flow cytometry, and immunoassays. Its isothiocyanate group reacts with primary amines, forming stable thiourea bonds for covalent attachment. Commonly applied in diagnostics, cellular imaging, and research to study molecular interactions, localization, and dynamics in biological systems.
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