Fluorescein5(6)-isothiocyanate

90%, 5- and 6-isomer mixture

Reagent Code: #127131
label
Alias Fluorescein isothiocyanate; 5(6)-fluorescein isothiocyanate, 4'(or 5')-fluorane isothiocyanate
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CAS Number 27072-45-3

blur_circular Chemical Specifications

scatter_plot Molecular Information
Weight 389.38 g/mol
Formula C₂₁H₁₁NO₅S
badge Registry Numbers
EC Number 248-207-7
MDL Number MFCD00005063
thermostat Physical Properties
Melting Point >360 °C(lit.)
inventory_2 Storage & Handling
Storage 2-8°C

description Product Description

Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology for tagging proteins, antibodies, and other biomolecules. It enables visualization and tracking of these molecules in techniques like fluorescence microscopy, flow cytometry, and immunoassays. Its isothiocyanate group reacts with primary amines, forming stable thiourea bonds for covalent attachment. Commonly applied in diagnostics, cellular imaging, and research to study molecular interactions, localization, and dynamics in biological systems.

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Test Parameter Specification
Appearance Light yellow to yellow to orange to brown powder to crystal
Purity (%) 89.5-100
Infrared Spectrum Conforms To Structure
NMR Conforms to Structure
Solubility (1 mg/mL, Acetone) Very Faint Yellow To Yellow And Very Faint Green-Yellow To Green-Yellow Liquid
Carbon (C) 54.1-71.0
Nitrogen (N) 3.0-4.0

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Size Availability Unit Price Quantity
inventory 1g
10-20 days ฿7,660.00
inventory 250mg
10-20 days ฿2,180.00
inventory 50mg
10-20 days ฿790.00
Fluorescein5(6)-isothiocyanate
Used extensively as a fluorescent labeling reagent in biochemistry and molecular biology for tagging proteins, antibodies, and other biomolecules. It enables visualization and tracking of these molecules in techniques like fluorescence microscopy, flow cytometry, and immunoassays. Its isothiocyanate group reacts with primary amines, forming stable thiourea bonds for covalent attachment. Commonly applied in diagnostics, cellular imaging, and research to study molecular interactions, localization, and dynamics in biological systems.
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