Fluorescein5(6)-isothiocyanate
96%, 5- and 6-isomer mixture
- Product Code: 127132
Alias:
FITC; fluorescein isothiocyanate; 5(6)-fluorescein isothiocyanate, 4'(or 5')-fluorane isothiocyanate
CAS:
27072-45-3
| Molecular Weight: | 389.38 g./mol | Molecular Formula: | C₂₁H₁₁NO₅S |
|---|---|---|---|
| EC Number: | 248-207-7 | MDL Number: | MFCD00005063 |
| Melting Point: | >360 °C(lit.) | Boiling Point: | |
| Density: | Storage Condition: | 2~8°C |
Product Description:
Fluorescein isothiocyanate (FITC) is widely used as a fluorescent dye in biological and chemical research. Its primary application is in labeling proteins, antibodies, and other biomolecules for fluorescence microscopy, flow cytometry, and immunofluorescence assays. FITC binds covalently to amino groups, enabling the visualization and tracking of specific molecules within cells or tissues. It is also employed in diagnostic tests, such as detecting antibodies or antigens in clinical samples. Additionally, FITC is used in environmental monitoring to trace water flow and contamination due to its strong fluorescence under UV light. Its versatility and high sensitivity make it a valuable tool in both research and industrial applications.
Product Specification:
| Test | Specification |
|---|---|
| Purity (HPLC) | 96-100 |
| Appearance | Yellow To Orange Powder |
| Fluorescence | Ex 492 Nm; Em 518 Nm In 0.1 M Phosphate Ph 8.0 |
| Infrared Spectrometry | Conforms To Structure |
Sizes / Availability / Pricing:
| Size (g) | Availability | Price | Quantity |
|---|---|---|---|
| 0.050 | 10-20 days | $41.11 |
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| 0.250 | 10-20 days | $149.50 |
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| 1.000 | 10-20 days | $406.98 |
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| 5.000 | 10-20 days | $1,244.21 |
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| 25.000 | 10-20 days | $5,647.94 |
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Fluorescein5(6)-isothiocyanate
Fluorescein isothiocyanate (FITC) is widely used as a fluorescent dye in biological and chemical research. Its primary application is in labeling proteins, antibodies, and other biomolecules for fluorescence microscopy, flow cytometry, and immunofluorescence assays. FITC binds covalently to amino groups, enabling the visualization and tracking of specific molecules within cells or tissues. It is also employed in diagnostic tests, such as detecting antibodies or antigens in clinical samples. Additionally, FITC is used in environmental monitoring to trace water flow and contamination due to its strong fluorescence under UV light. Its versatility and high sensitivity make it a valuable tool in both research and industrial applications.
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