Fluorescein5(6)-isothiocyanate

96%, 5- and 6-isomer mixture

  • Product Code: 127132
  Alias:    FITC; fluorescein isothiocyanate; 5(6)-fluorescein isothiocyanate, 4'(or 5')-fluorane isothiocyanate
  CAS:    27072-45-3
Molecular Weight: 389.38 g./mol Molecular Formula: C₂₁H₁₁NO₅S
EC Number: 248-207-7 MDL Number: MFCD00005063
Melting Point: >360 °C(lit.) Boiling Point:
Density: Storage Condition: 2~8°C
Product Description: Fluorescein isothiocyanate (FITC) is widely used as a fluorescent dye in biological and chemical research. Its primary application is in labeling proteins, antibodies, and other biomolecules for fluorescence microscopy, flow cytometry, and immunofluorescence assays. FITC binds covalently to amino groups, enabling the visualization and tracking of specific molecules within cells or tissues. It is also employed in diagnostic tests, such as detecting antibodies or antigens in clinical samples. Additionally, FITC is used in environmental monitoring to trace water flow and contamination due to its strong fluorescence under UV light. Its versatility and high sensitivity make it a valuable tool in both research and industrial applications.
Product Specification:
Test Specification
Purity (HPLC) 96-100
Appearance Yellow To Orange Powder
Fluorescence Ex 492 Nm; Em 518 Nm In 0.1 M Phosphate Ph 8.0
Infrared Spectrometry Conforms To Structure
Sizes / Availability / Pricing:
Size (g) Availability Price Quantity
0.050 10-20 days $49.72
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0.250 10-20 days $180.81
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1.000 10-20 days $492.21
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5.000 10-20 days $1,504.77
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25.000 10-20 days $6,830.74
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Fluorescein5(6)-isothiocyanate
Fluorescein isothiocyanate (FITC) is widely used as a fluorescent dye in biological and chemical research. Its primary application is in labeling proteins, antibodies, and other biomolecules for fluorescence microscopy, flow cytometry, and immunofluorescence assays. FITC binds covalently to amino groups, enabling the visualization and tracking of specific molecules within cells or tissues. It is also employed in diagnostic tests, such as detecting antibodies or antigens in clinical samples. Additionally, FITC is used in environmental monitoring to trace water flow and contamination due to its strong fluorescence under UV light. Its versatility and high sensitivity make it a valuable tool in both research and industrial applications.
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