AT7867 is primarily used in biomedical research as a potent and selective inhibitor of the protein kinases Akt and PDK1, which play key roles in cell survival, proliferation, and metabolism. It is widely applied in studies related to cancer signaling pathways, particularly to investigate the PI3K/Akt/mTOR pathway, which is frequently dysregulated in various cancers. Researchers use AT7867 to suppress tumor cell growth and induce apoptosis in cancer cell lines, making it a valuable tool for preclinical studies in oncology. Additionally, it has been employed to explore insulin signaling and glucose metabolism, offering insights into metabolic disorders such as diabetes. Its ability to cross the blood-brain barrier also makes it useful in neuroscience research, particularly in models of neurodegeneration and brain cancer.