Apcin is primarily used in research settings as a small molecule inhibitor of the anaphase-promoting complex/cyclosome (APC/C), a key regulator of cell cycle progression. By inhibiting APC/C, Apcin helps stabilize cell cycle substrates that are normally targeted for degradation, making it a valuable tool in studying mitosis and cell division. It is often used in combination with other inhibitors, such as proTAME, to achieve more potent and sustained cell cycle arrest in experimental models. This application is particularly useful in cancer research, where understanding cell cycle control can inform the development of novel anti-mitotic therapies. Apcin’s ability to block the recognition of specific substrates by APC/C also makes it instrumental in dissecting the molecular mechanisms of ubiquitin-mediated proteolysis.