Used primarily as a photoaffinity labeling agent in biochemical and pharmacological research. Upon exposure to UV light, it generates highly reactive carbene species that form covalent bonds with nearby biomolecules, enabling the study of molecular interactions in proteins, enzymes, and receptor-ligand systems. Its azido group can also participate in click chemistry reactions, facilitating conjugation with fluorescent tags or affinity probes. This dual functionality makes it valuable for mapping binding sites, identifying unknown targets, and developing diagnostic or therapeutic probes in complex biological environments.