Used in research and diagnostic applications to detect histone H4, particularly in chromatin immunoprecipitation (ChIP) assays, immunofluorescence, and Western blotting. Helps study epigenetic modifications, gene regulation, and nuclear structure. Valuable in autoimmune disease research, including systemic lupus erythematosus (SLE), where anti-histone antibodies are biomarkers. Also employed in the development and validation of immunoassays targeting nucleosomal components. Its specificity supports accurate detection of histone H4 in complex biological samples.