Used in research and diagnostic laboratories to measure the activity of 17β-hydroxysteroid dehydrogenase (17β-HSD) in fish samples. This enzyme plays a key role in steroid metabolism, particularly in the biosynthesis and regulation of sex hormones like testosterone and estradiol. The kit enables endocrine disruption studies, helping assess the impact of environmental pollutants such as endocrine-disrupting chemicals (EDCs) on fish hormonal systems. It is widely applied in ecotoxicology to evaluate the health of aquatic ecosystems and to monitor the effects of industrial, agricultural, and pharmaceutical contaminants on fish reproduction and development. The assay supports high-throughput analysis and provides a sensitive, quantitative method for detecting changes in enzyme activity linked to hormonal imbalances.