Fosfomycin disodium salt is primarily used as an antibiotic in clinical and research settings. It is effective against a broad range of Gram-positive and Gram-negative bacteria, including multidrug-resistant strains. Its main application lies in treating urinary tract infections (UTIs) due to its high concentration in urine and ability to penetrate bacterial cells effectively. The compound works by inhibiting early stages of bacterial cell wall synthesis, making it bactericidal. It is often used in hospitals for treating infections caused by resistant pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) and extended-spectrum beta-lactamase (ESBL)-producing organisms. In research, fosfomycin disodium salt is used to study antibiotic resistance mechanisms and to develop combination therapies that enhance its efficacy. It is also employed in microbiology labs for bacterial selection and susceptibility testing. Due to its unique mechanism and low cross-resistance with other antibiotics, it is a valuable option in stewardship programs aiming to preserve antibiotic effectiveness.