Used in biomedical research to measure PHD (prolyl hydroxylase domain) enzyme activity in human samples. Helps assess hypoxia-related pathways, as PHD enzymes regulate HIF (hypoxia-inducible factor) stability. Commonly applied in studies of cancer, cardiovascular diseases, and metabolic disorders. Enables high-throughput screening of compounds that modulate PHD activity, supporting drug development. Provides quantitative data from cell lysates, tissue extracts, or serum, making it valuable for both basic research and clinical biomarker studies.