Used to detect and quantify the activity of human β-trypsin in biological samples such as serum, plasma, or cell culture supernatants. Commonly applied in clinical research to study pancreatic function and diseases like pancreatitis, cystic fibrosis, and other digestive disorders. Helps in monitoring disease progression and evaluating treatment efficacy by providing a sensitive and specific measurement of active trypsin levels. Also used in diagnostic laboratories and drug development studies involving protease activity.