Used in biomedical research to quantify levels of human α-enolase, an enzyme involved in glycolysis and implicated in autoimmune diseases, cancer progression, and inflammation. The ELISA kit enables detection of α-enolase in serum, plasma, cell lysates, and other biological samples, supporting studies on tumor biomarkers, autoimmune responses (such as in rheumatoid arthritis or lupus), and infectious disease mechanisms where α-enolase acts as a plasminogen receptor on cell surfaces. Its high sensitivity and specificity make it valuable for clinical diagnostics and therapeutic monitoring in research settings.