Used as a fluorescent probe in biochemical and cellular research due to its ability to bind to specific proteins and accumulate in mitochondria. It is particularly valuable for studying mitochondrial membrane potential and tracking mitochondrial dynamics in live cells. Its fluorescence properties make it suitable for imaging applications, including confocal microscopy and flow cytometry. Also employed in apoptosis studies, where changes in mitochondrial structure and function are monitored during programmed cell death. The compound’s cell-permeable nature and selective organelle targeting enhance its utility in cell biology and pharmacological research.