Used as a substrate in enzymatic assays for detecting β-galactosidase activity. The compound releases 4-methoxyphenol upon hydrolysis, which can be easily measured spectrophotometrically. Commonly applied in biochemical research and diagnostic procedures to monitor enzyme kinetics and inhibition. Also utilized in studies involving glycosidase mechanisms and in the development of biosensors. Its stability and clear detection signal make it suitable for high-throughput screening in pharmaceutical and biotechnological applications.