Used as a fluorescent dye for staining cell membranes in live-cell imaging, particularly in yeast and fungal cells. It allows visualization of membrane dynamics such as endocytosis, exocytosis, and vesicle trafficking due to its bright fluorescence and strong binding to lipid bilayers. Commonly applied in studies of cellular uptake, organelle labeling, and plasma membrane behavior during cell division or stress. Its red fluorescence makes it suitable for use in multicolor labeling experiments when combined with green fluorescent probes. Also used in high-throughput screening and microscopy techniques where photostability and low cytotoxicity are required.