Used in biochemical research as a dipeptide analog to study enzyme activity, particularly in relation to peptidases and proteases that recognize proline-containing sequences. Serves as a building block in the synthesis of larger peptides designed to mimic natural substrates or inhibitors. Also employed in the development of assays for drug discovery targeting metabolic pathways involving collagen degradation or neuropeptide processing. Its structure supports investigations into proline-dependent conformational changes in proteins and peptides.