WAY 316606 hydrochloride is primarily used in scientific research as a selective inhibitor of secreted frizzled-related protein 1 (sFRP-1). By inhibiting sFRP-1, it enhances Wnt signaling, a key pathway involved in bone formation and tissue regeneration. This makes it a valuable tool in studies focused on osteogenesis and bone repair, particularly in models of osteoporosis or fracture healing. Researchers use it to investigate the potential of modulating Wnt signaling to promote stem cell differentiation into osteoblasts, supporting the development of regenerative therapies for bone diseases. Its application remains confined to preclinical research and is not used in clinical or industrial settings.