Wiskostatin is primarily used as a selective inhibitor of the Wiskott-Aldrich syndrome protein (WASP) family, making it a valuable tool in cellular and molecular biology research. It is applied in studies involving actin cytoskeleton dynamics, as it effectively suppresses WASP-mediated actin polymerization. This allows researchers to investigate the role of actin assembly in processes such as cell motility, endocytosis, and intracellular pathogen movement. Wiskostatin has been utilized in experiments with immune cells to probe the function of WASP in T-cell activation and dendritic cell migration. Due to its ability to cross cell membranes, it is suitable for live-cell imaging and functional assays where temporal control of actin regulation is required. Its application supports the development of targeted therapies for immune disorders and cancers linked to aberrant actin regulation.