Used in biomedical research to quantify levels of Human Centromere Protein B (CENP-B) in biological samples such as serum, plasma, or cell lysates. The ELISA kit enables detection and measurement of CENP-B autoantibodies, which are associated with certain autoimmune disorders, particularly in the diagnosis of CREST syndrome (a subtype of systemic sclerosis). It supports studies on chromosomal stability, centromere function, and autoimmune response mechanisms. The assay is widely used in clinical research and drug development for evaluating immune reactivity and monitoring disease progression related to anti-centromere antibodies.