Used in biomedical research to quantify levels of High Mobility Group Protein B1 (HMGB1) in biological samples such as serum, plasma, and cell culture supernatants. This ELISA kit is particularly valuable in studies related to inflammation, sepsis, autoimmune diseases, and cancer, where HMGB1 acts as a key mediator of immune response and tissue damage. Researchers use it to monitor disease progression, evaluate therapeutic efficacy, and investigate the role of HMGB1 in cellular signaling pathways. The assay enables sensitive and specific detection, supporting both clinical research and drug development.