Used in biomedical research to quantify levels of GPNMB protein in cell lysates, tissue extracts, and biological fluids such as serum or plasma. Enables researchers to study the role of GPNMB in processes like melanoma progression, inflammation, and tissue repair. Commonly applied in cancer research, particularly to assess GPNMB expression in non-metastatic versus metastatic melanoma cells. Also utilized in drug development to monitor target engagement and evaluate therapeutic efficacy of anti-GPNMB agents. Supports studies in neurodegenerative diseases and osteoarthritis due to GPNMB's involvement in cellular differentiation and immune modulation. Provides a reliable, sensitive, and specific method for high-throughput screening in laboratory settings.