Used in life science research to detect and quantify proteins fused with the FLAG-tag, a synthetic peptide tag commonly engineered into recombinant proteins. The kit enables researchers to measure expression levels, monitor protein purification, and assess protein-protein interactions in cell lysates or biological samples. Widely applied in drug development, molecular biology, and biotechnology for confirming successful protein expression and optimizing transfection efficiency. The assay offers high specificity and sensitivity, allowing accurate detection of low-abundance FLAG-tagged proteins without interference from native cellular proteins.