Used in biochemical and medical research to detect and quantify homogentisic acid levels in biological samples. Helps in studying metabolic disorders such as alkaptonuria, a rare genetic condition caused by the deficiency of homogentisase enzyme. The kit enables accurate measurement of enzyme activity in plant and tissue extracts, supporting research in metabolic pathways, oxidative stress, and pigment formation. Also utilized in pharmaceutical development for monitoring therapeutic efficacy and in diagnostic laboratories for early detection of metabolic abnormalities.