Used in biomedical research to measure levels of the CYP27B1 enzyme in human tissue samples and cell cultures. This enzyme plays a key role in vitamin D metabolism by converting 25-hydroxyvitamin D to its active form, 1,25-dihydroxyvitamin D. The ELISA kit enables researchers to study CYP27B1 expression in various diseases such as chronic kidney disease, autoimmune disorders, and cancer. It is also used in drug development to assess how new compounds affect vitamin D activation in the body. The assay provides a sensitive and specific method for quantifying protein levels in serum, plasma, and tissue lysates, supporting studies on endocrine function and metabolic regulation.