Used in research settings to detect and quantify nicarbazin residues in biological samples such as eggs, tissues, and feed. The ELISA kit enables high-throughput screening, making it valuable for monitoring compliance with veterinary drug regulations and ensuring food safety. It is widely applied in agricultural laboratories, quality control centers, and residue monitoring programs to prevent contamination in the food chain. The method offers high sensitivity and specificity, allowing accurate detection at low residue levels.