Used in biomedical research to detect and quantify 5-nucleotidase enzyme activity in chicken samples, particularly in studies related to liver function, metabolic disorders, and purine metabolism. The ELISA format allows for high-throughput analysis in laboratories focusing on avian health, poultry disease diagnostics, and physiological research. It is commonly applied in veterinary research settings to assess tissue damage or immune responses where 5-NT serves as a biomarker. The kit enables precise measurement in serum, plasma, or tissue homogenates, supporting studies on enzyme regulation and pathophysiological conditions in poultry.