Used in biological research to detect and quantify Zonula Occludens-1 (ZO-1) protein levels in chicken samples. This kit is particularly applied in studies related to tight junction integrity in epithelial and endothelial cells, helping assess gut health, intestinal barrier function, and the impact of pathogens or toxins in poultry. It supports research in veterinary science, immunology, and avian disease mechanisms, especially in conditions affecting cellular permeability and inflammation. The assay enables high-throughput analysis in experimental models investigating nutritional, infectious, or environmental effects on barrier tissue function in chickens.